Description: A Novel Cre Recombinase Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary Application.- Vaccination with Messenger RNA - a Promising Alternative to DNA Vaccination.- Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency.- CpG Oligonucleotides as Vaccine Adjuvants.- Molecular Adjuvants for DNA Vaccines: Application, Design, Preparation and Formulation.- Assessing Antigen-specific Cellular Immune Responses Upon HIV/SIV Plasmid DNA Vaccination in the Non-human Primate Model.- Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design.- Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation.- Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic Monoliths.- Purification of Plasmid DNA by Multimodal Chromatography.- Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment.- Tumor-Specific CD8+ T Cell Responses Induced by DNA Vaccination.- Therapeutic DNA Vaccine Against HPV16-Associated Cancer.- Bulk and Microfluidic Synthesis of Stealth and Cationic-Liposomes for Gene Delivery Applications.- Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field.- Main Features of DNA Based Vectors for Use in Lactic Acid Bacteria and Update Protocols.- Ethics of DNA Vaccines Transfer for Clinical Research.- Preparation of an Academic Clinical Trial.

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