Description: 1. Overview of G proteins (GTP binding proteins) in Eukaryotes 1.1 Overview 1.2 Small G-protein structure and Domain features 1.3 Biochemical regulation of small GTPases 1.4 GEFs 1.5 GAPs 1.6 GDIs 1.7 Localization and Post-translational modifications References 2. Overview of Small GTPase Signaling Proteins in Plants 2.1 Abstract 2.2 Introduction 2.3 Small GTPase Complement in Plantae 2.4 Plant Specific Functions of ROPs References 3. Identification and Classification of Rho GTPases in Plants 3.1 Introduction 3.2 Nomenclature 3.3 ROP GTPase complement in Arabidopsis 3.4 ROP GTPase complement in rice References 4. Sequence, Structure, and Domain Analysis of GTPases in Plants 4.1 Introduction 4.2 Domain Identification and Confirmation of Rice Small GTPases 4.3 Multiple alignment and phylogenetic analysis 4.4 Comparative Phyletic analysis of Rice Small GTPase genes 4.5 Gene nomenclature and localization of small GTPases 4.6 G-Domain Conservation Pattern References 5. Expression of Small GTPases Under Stress and Developmental Conditions in Plants 5.1 Expression Pattern of OsGTPases in Abiotic Stress 5.2 Expression Pattern of OsGTPases in Developmental Stages 5.3 Expression Pattern of OsGTPases during Phytohormone treatment 5.4 Expression Pattern of AtGTPases in Devlopmental stages 5.5 Expression Pattern of AtGTPases in Abiotic Stress 5.6 Expression Pattern of AtGTPases during Phytohormone treatment References 6. Emerging Roles of Rho GTPases in Plants 6.1 Rho GTPases: Versatile signaling molecules in plants 6.2 Rho Activates plant defense mechanisms 6.3 Role in intracellular trafficking and cell polarity 6.4 Role in pollen tube growth 6.5 Role in root hair development 6.6 Small GTPases Controls Cell Morphogenesis References 7. Cellular Localization of Small GTPases 7.1 Membrane association of RAC/ROP GTPase 7.2 Hypervariable Region regulate RAC/ROP localization 7.3 Post-translational Lipid Modifications determines the ROP Activity 7.4 Significance of Subcellular localization in ROP Signaling References 8. Functional Genomic Perspective of Small GTPases 8.1 Regulatory mechanism of Rho signaling 8.1.1 Regulators and Effectors of ROP 8.1.2 ROP interactive CRIB domain containing proteins (RICs) 8.1.3 Two counteractive pathways coordinate the actin dynamics in pollen tube elongation 8.1.4 The antagonistic ROP2/RIC1 pathway promote microtubule organization 8.2 Unconventional effectors of ROP/RAC pathways References 9. Systemic Approaches to Resolve Spatio-Temporal Regulation of GTPase Signaling 9.1 Introduction 9.2 Detection of Rho GTPase activity in plant cells 9.3 FRET (fluorescence resonance energy transfer) assay 9.4 Biochemical assay for ROP activity detection 9.5 Light-gated protein interaction 9.6 Significance of Systemic Approaches to Measure Signaling Modularity References 10. Key Questions and Future Prospects 10.1 Introduction 10.2 GTPase and lipid interaction 10.3 Upstream and downstream regulators 10.4 Future perspectives References

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